Combined Physio-Biochemical and Transcriptome Analyses Illuminate the Resistance Response of Rice Priming with Decoyinine against Nilaparvata lugens

The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a notorious pest in rice production. microbial secondary metabolite, decoyinine (DCY), produced by Streptomyces hygroscopicus. Recent studies found that seed priming with DCY could enhance resistance to BPH and Laodelphax striatellus; however, the mechanism of enhancing insect remains unclear. Here, an integrated physio-biochemical transcriptome analysis was performed on after infestation. Defense-related enzymes activities such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione S transferase (GST), polyphenol oxidase (PPO), concentrations hydrogen peroxide (H2O2), callose, free amino acids were significantly increased than control plants Concentrations soluble sugars, sucrose, glucose higher treated infestation; concentration malondialdehyde (MDA) decreased In analysis, GO functional annotation KEGG pathway enriched defense response, transcription factors, metabolites, reactive oxygen species, cell wall organization these data also support results. qRT-PCR results further verified differential expressed genes related DCY-treated responding BPH. Meanwhile, it indicated might regulating WRKY factor genes. Our provide basis for exploring molecular response against infestation valuable resources pests.